IMPACTT Bioinformatics Workshop

About the Workshop

Our workshops in 2022 and 2023 provided an introduction to marker-gene analysis, metagenomic, and metatranscriptomic data analysis followed by hands-on practical tutorials for each session to demonstrate the use of relevant bioinformatics and statistical tools. Modules consisted of lectures covering both theoretical and practical components followed by hands-on bioinformatic tutorials guided by instructors and teaching assistants.

Participants gained practical experience and skills in:

  • Designing appropriate microbiome-focused experiments
  • Understanding the advantages and limitations of metagenomic data analysis
  • Devising an appropriate bioinformatics workflow for processing and analyzing microbiome sequence data (marker-gene, shotgun metagenomic, and metatranscriptomic data)
  • Applying appropriate statistics to undertake rigorous data analysis

Upcoming workshops

Upcoming bioinformatics workshops are organized by Bioinformatics.ca.
Head to their Canadian Bioinformatics Workshops page for a full list of current workshops.

Past workshops

University of Calgary
July 5-7, 2023
See the schedule

McGill University
December 6-7, 2022
See the schedule

University of Calgary
May 30 – June 1, 2022
See the schedule

Workshop Resources

Lecture Videos

We recorded the lectures from our May 2022 workshop and have made them available on our YouTube channel. Please note that our lectures were recorded live during our in-person workshop and may have audio and technical issues. Thank you to our instructors and TAs for putting together and teaching the lectures.

Instructors

Dr. Will Hsiao (Simon Fraser University)
Dr. Rob Beiko (Dalhousie University)
Dr. Morgan Langille (Dalhousie University)
Dr. Laura Hug (University of Waterloo)
Dr. John Parkinson (University of Toronto)

TAs and Technical Assistance

Diana Haider (Dalhousie University)
Dr. Robyn Wright (Dalhousie University)
Nikhil George (University of Waterloo)
Ana Popovic (University of Toronto)
Dr. Hena Ramay (University of Calgary)
Zhibin Lu (HPC4Health)

Lecture & Tutorial Slides

Our tutorial sessions were not recorded, but we have made our tutorial slides publicly available so you can try them out on your own. Thank you to our instructors and TAs for putting together and teaching the tutorials.

Lecture Slides

Module 1: Introduction to Microbiome Studies
Module 2: Marker Gene Taxonomic Analysis
Module 3: Metagenome Taxonomic Analysis
Module 4: Metagenome Functional Annotation
Module 5: Metagenome Assembly & MAGs
Module 6: Transcriptomics
Special Topic: Sample Collection & Prep

Tutorial Slides

Module 1: Logging Into AWS
Module 2: Marker Gene Taxonomic Analysis
Module 3: Metagenome Taxonomic Analysis
Module 4: Metagenome Functional Annotation
Module 5: Metagenome Assembly & MAGs
Module 6: Transcriptomics

AWS Instance

We have made our AWS instance publicly available to go along with the tutorials. Follow the instructions below to access. Thank you to Zhibin Lu at HPC4Health for setting up our AWS instance.

  1. Follow the Amazon instructions on launching an EC2 instance from a custom Amazon Machine Image (AMI)
  2. AWS Region: us-east-1 (N. Virgina)
  3. AMI type: public image
  4. AMI name: mic_221203
  5. AMI ID: ami-03a45ac0d19c46d15
  6. For AWS accounts and other settings, refer to AWS documentation

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