New Paper: Questioning the fetal microbiome illustrates pitfalls of low-biomass microbial studies
Congratulations to our Platform 2 Co-Lead Dr. Marie-Claire Arrieta on this recent publication! Abstract Whether the human fetus and the prenatal intrauterine environment (amniotic fluid and placenta) are stably colonized by microbial communities in a healthy pregnancy remains a subject of debate. Here we evaluate recent studies that characterized microbial populations in human fetuses from […]
IMPACTT Microbiome Standards & Benchmarking
IMPACTT Microbiome Standards & Benchmarking IMPACTT Microbiome Standards What’s in our standards? A community of 45 bacterial species that are phylogenetically diverse and evenly distributed Novel, sequenced strains Encased in human fecal matrix (3 types) or PBS (1 type) Watch our video to learn more Fill out the form and get the standards What makes […]
Introducing: IMPACTT Microbiome Standards
We are excited to introduce our IMPACTT Microbiome Standards! A defined microbial community of 45 phylogenetically diverse, evenly distributed bacteria encased in human fecal matrix or PBS. The best part: it’s free for Canadian labs! This initiative is led by our Platform 4 Lead Dr. Laura Sycuro (University of Calgary), who partnered with Dr. Emma […]
Protocol: Generation, Maintenance, and Monitoring of Gnotobiotic Mice
Summary Gnotobiology has revolutionized the study of microbiota-host interactions. This protocol explains how to generate, maintain, and monitor gnotobiotic mice. Three monitoring methods are presented and compared: bacterial culture, microscopy to visualize the presence (or absence) of bacteria using Gram staining or DNA staining, and 16S rRNA gene amplification and sequencing. The generation and maintenance […]
New Paper: Innate Responses to Gut Microbiota; Critical Assessment of the Necessary Experimental Controls
Congratulations to our Director and Platform 1 Lead Dr. Kathy McCoy on this recent publication! Focusing on the recent findings (since 2017) regarding the commensal bacteria of the mouse intestinal tract, Dr. McCoy and Dr. Ohland discuss the importance and advances of what experimental controls are required to reliably determine how the microbiota influences the […]
New Paper: PSORTm : A Bacterial and Archaeal Protein Subcellular Localization Prediction Tool for Metagenomics Data
Congratulation to our Platform 5 Co-Lead Dr. Fiona Brinkman on this recent publication! Abstract We report PSORTm, the first bioinformatic tool designed for prediction of diverse bacterial and archaeal protein subcellular lo from metagenomics data. PSORTm incorporates components of PSORTb, one of the most precise and widely used protein SCL predictors, with an automated classification […]
